Journal: Scientific Reports

Article Title: Chemical hybridization of sulfasalazine and dihydroartemisinin promotes brain tumor cell death

doi: 10.1038/s41598-021-99960-z

Figure Lengend Snippet: Morphologic profiling of Sulfasalazine, DHA and the novel hybrid compound AC254 on rodent glioma cells. ( A ) Rat glioma cells (F98) were treated with a range of concentrations of SAS, DHA, AC254, SAS and solvent DMSO combined with DHA. Cell morphology was examined after 72 h with light microscopy. Olympus × 71 with cell F-Software (Olympus). Scale bar, 200 µM. ( B ) F98 were treated with 0.5 µM of SAS, DHA, AC254 and SAS combined with. Cells were fixated and stained with actin marker Phalloidin 488 and DNA marker Hoechst after 24 h of treatment. Axio Observer with Zen Software (Zeiss). Scale bar: 20 µm.

Article Snippet: Olympus × 71 with cell F-Software (Olympus).

Techniques: Light Microscopy, Software, Staining, Marker

Journal: Scientific Reports

Article Title: Chemical hybridization of sulfasalazine and dihydroartemisinin promotes brain tumor cell death

doi: 10.1038/s41598-021-99960-z

Figure Lengend Snippet: Effects on human cell viability (U87, TN22). ( A ) Cells (U87) were treated with 1 µM of the compounds. Cell viability was measured after 72 h. n = 4. * indicates significance to control ( p < 0.05). ( B ) U87 were treated with various concentrations of the compounds. Cell viability was measured the same way as in A. n = 4. * indicates significance to control ( p < 0.05). ( C ) TN22 were treated with 5 µM of the compounds. Cell morphology was examined after 72 h with light microscopy. Olympus × 71 with cell F-Software (Olympus). Scale bar, 200 µm. ( D ) TN22 were treated with 5 µM of the compounds, cell viability was measured after 72 h. n = 4. * indicates significance to control ( p < 0.05).

Article Snippet: Olympus × 71 with cell F-Software (Olympus).

Techniques: Light Microscopy, Software

Journal: Scientific Reports

Article Title: Chemical hybridization of sulfasalazine and dihydroartemisinin promotes brain tumor cell death

doi: 10.1038/s41598-021-99960-z

Figure Lengend Snippet: Impact on human cell morphology. ( A ) Human cells (U87) were treated with a variation of concentrations of SAS, DHA, their covalently bound hybrid (AC254) and their 1:1 mixture. Cell morphology was examined after 72 h with light microscopy. Olympus × 71 with cell F-Software (Olympus). Scale bar, 200 µM. ( B ) Cells (U87) were treated with 5 µM of SAS, DHA, AC254 and SAS combined with. Cells were fixated and stained with actin marker Phalloidin 488 and DNA marker Hoechst after 24 h of treatment. Axio Observer with Zen Software (Zeiss). Scale bars represents 20 µm.

Article Snippet: Olympus × 71 with cell F-Software (Olympus).

Techniques: Light Microscopy, Software, Staining, Marker

Journal: Scientific Reports

Article Title: Chemical hybridization of sulfasalazine and dihydroartemisinin promotes brain tumor cell death

doi: 10.1038/s41598-021-99960-z

Figure Lengend Snippet: Impact of the compounds on cell migration. ( A , B , C ) A scratch was made into plated cells (F98) after 36 h. Cells were then treated with 0.5 µM ( A ) and 5 µM ( B , C ) of the compounds. Representative images were taken (Olympus × 71 with cell F-Software (Olympus)). ( B ), quantified and related to the time the scratch was made (0 h) ( A , C ). n = 3. * indicates significance to control ( p < 0.05).

Article Snippet: Olympus × 71 with cell F-Software (Olympus).

Techniques: Migration, Software